Анализатор плоидности PA
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Автоматический анализатор степениплоидности или анеуплоидности растительных клеток
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DNA histogram of a mixture of nuclei from leaf tissue of a diploid and a triploid sugar-beet (Partec CyStain UV, UV excitation). The peak positions on the X-axis correspond to the ploidy level, the first peak represents the diploid, the second peak represents the triploid nuclei. The peaks 3 and 4 contain the DNA values of the endomitotic nuclei (4 n, 6 n). The results of the automatic peak evaluations appear immediately after each measurement (peak position, cell number per peak, %, peak width: CV). | Peak positions in the DNA histograms of 50 sugar beet seedlings (100% corresponds to 27 chromosomes: triploid). 8 seedlings of this sample (16%) show aneuploid DNA values (26 resp. 28 chromosomes). |
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Принцип измерения |
Procedure for fresh or frozen plant material |
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Ploidy determinations with the Partec PA Ploidy Analyser are perФормаed by the precise measurement of the total DNA content of individual nuclei, which can be derived from almost any plant tissue. |
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Анализа плоидности в PA |
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