Particle Analysing System PAS-III
Modular Flow Cytometer with all Options
 | 2 or 3 lasers (e.g. Argon-ion, He-Ne, NdYAG or UV laser) and HBO lamp |
| Sensitivity:<100 FITC molecules |
| True volumetric absolute counting (e.g. CD 34+ , CD4/CD8 and other immunolabelled cells without need for reference particles) |
| 1-8 optical parameters (FSC,SSC, FL1-FL6) and time parameter for kinetic studies |
| 16 bit ADC for each parameter |
| Video flow monitor |
| 32 bit Windows™ operation system |
| 32 bit Windows™ FloMax® software |
| Complete software package for lymphocyte subset, cell cycle, apoptosis, ploidy, microbiology, bacteria and yeast analysis |
| Ethernet connection |
| Built-in active LCD screen |
| Optional external monitor |
| Processor > 1.7 GHz |
| Interchangeable filter combinations |
| Real time data acquisition and display |
| Automated immunology sample preparation and analysis |
| BarКод reader |
| 650 MB CD-Writer for data backup |
| Partec PAS-III |
Specifications: 1. Systems |
| Size | Computer housing: 44 cm L x 61 cm W x 47 cm H Optical housing: 75 cm L x 61 cm W x 47 cm H Laser Power Supply: 35 cm L x 34 cm W x 15 cm H Sheath fluid / waste bottle rack: 33 cm L x 13 cm W x 34 cm H Keyboard: 47 cm L x 20 cm W x 4 cm H |
| Applications | Immunophenotyping, DNA analysis (Cell cycle, ploidy, apoptosis), Microbiology (bacteria and yeast analysis) 1 to 6 colour analysis. True volumetric absolute counts = counting per volume. |
| True Absolute Counting | Based on precise counting and fluid volume
measurement. No need for reference sample or beads. |
| Instrument Check |
Partec DNA Control UV QC particles Partec CountCheck QC beads |
| Sample Preparation Accessory | Sample Automate for 36 sample tubes: Lyse no wash method, standard lyse / wash method Preparation with up to 16 different reagents |
| Set-up Time | Max. 5 minutes |
| No. of Parameters | Up to 8 optical parameters (FSC, SSC, FL1-FL6), time parameter for kinetic measurements, pulse height, pulse area, pulse width for each parameter |
| Particle Size Range | 0.2 мкл - 200 мкл |
| Maximum acquisition speed | 30,000 events/sec |
| Acquisition Stop Time | Time-, event- or volume-based |
| Trigger | On all parameters or on specific trigger parameters, selectable in software |
| Data Resolution | 65,536 channels (16 bit) |
| Service | 1-3 years service contracts |
| Warranty | 12 months on all parts except filters, mirrors, other quartz or glass parts, disposables and cuvettes |
| Partec PAS-III |
Specifications: 2. Optics |
| Laser / Output | 25 mW, 488 nm, air cooled argon-ion laser красный Diode Laser 25mW at 635 nm, temeprature controlled зеленый Diode Laser NdYAG 50mW at 532nm (optional) Blue Diode Laser 490 nm (optional) 100W HBO lamp (UV-excitation) |
| Dual Light Source System |
Argon ion laser and HBO lamp / 25mW 635nm laser diode Allows to use the dyes, excitable with 488 nm and UV light Argon ion laser and красный laser diode |
| Detectors | 1 to 8 (FSC, SSC, FL1, FL2, FL3, FL4, FL5, FL6) |
| Filters | Changeable set of preassembled filter combination blocks or single optical elements |
| Optical Coupling | UV transmitting quartz optics / high sensitivity laser objective High numerical aperture air objectives Optional: High numerical aperture immersion coupling, for e.g. detection of weak cytokines |
| Spatial Beam Separation | Laser / lamp by insertion of diaphragms, adjustable according to experimental needs |
| Excitation Optics | Elliptical 10 мкл x 100 мкл at 488 nm Other beam geometries upon request HBO lamp: Köhler illumination |
| Partec PAS-III |
Specifications: 3. Computer System |
| Computer System | Processor > 1.7 GHz Built-in-Ethernet >256 MB RAM |
| Standard Data Storage | >20.5 GB Hard disk, 3.5 in. floppy disk drive, 650 MB rewritable CD (CD-RW) |
| Optional Storage | 100 MB Iomega ZIP other drives can be connected upon request |
| Printer |
DeskJet, colour printer Laser Jet 6 (b&w laser printer) optional other printers upon request support for all other printers by Windows printing via network |
| Data Entry | Full keyboard / barКод reader / Мышиные |
| Partec PAS-III |
Specifications: 4. Fluidics |
| Sample Delivery | Computer controlled precision syringe pump for
sample, built-in air pressure for sheath fluid |
| Sampling Volume |
Continuous up to 1500 µl 200 µl for precision absolute counting Syringe count method for free sampling volume Other counting volumes upon request |
| Flow Rates (Sample Uptake) | 1) Sample volume speed adjustable continuously between 0 and 1200 µl / min 2) Sheath fluid pressure continuously adjustable |
| Fluidics Volume | 2 x 5-litre reservoirs for sheath fluid and waste |
| Fluid Level Indicators | For full waste, low sheath fluid |
| BioSafety System | Avoids sample droplets and sample cross contamination (computer controlled) |
| Partec PAS-III |
Specifications: 5. Software |
| Software | 32 bit Windows™ FloMax® software for routine and research applications, with integrated Realtime acquisition software, Time parameter for kinetic studies, Multiparameter N-colour compensation, (online and offline) Multiparameter gating, MultiColor gating, Peak and cluster analysis and statistics, Ratio measurements Parameter arithmetics DNA-cell cycle analysis, DNA peak analysis, Sample automat control Report module: automated multi-tube report generation as MS Word document, Copy & paste to desktop publishing software |
| Optional Software | Other software upon request |
| Lymphocyte Gating |
Light-scatter gating or fluorescence gating Gates are set once and saved on harddisk |
| Acquisition Gating | Lower-/upper-level hardware thresholds for event triggering parameters, adjusted by software Real-time software gating: Regions, quadrants, 1P-ranges 32 regions can be combined to 32 gates in free logical combinations Regions can be defined for 1 or 2 parameter histograms |
| Gating / Crosstalk Compensation |
On- or offline gating and crosstalk compensation provide adjustments without need to rerun samples Different gating and compensation adjustments can be stoкрасный and reload |
| Protocols | Individual instrument setups and acquisition displays can be saved in setup-files The plot display, axis labels, statistics and cell concentrations are saved in documents together with the acquisition data |
| Panels | Multi-tube analysis can be pкрасныйefined in panels Automated panel acquisition |
| Report Generation | Report module for Microsoft Word, directly called by the FloMax software. Single- or Multi-Page Report templates include institute logo(s), addresses, graphs and statistical results. Templates can be individually designed in a desktop publishing manner. Individual calculations can be defined in spreadsheet Формаulas. Copy & Paste / export to desk-top publishing software. Multi-page - Multitube reports |
| Document Plots | 1 - 8 fixed plots per page Multi-page reports in Microsoft Word with different sizes and any number of plots can be individually arranged by the user |
| Data Parameters Stoкрасный | All parameters stoкрасный in FCS 2.0 standard listmode Формаat. |
| Printer Support | All printers supported by Windows Printing queue Network printing Print to file |
| Files | Can be accessed through the network Windows search engine allows search of files and folders throughout the network |
